PTEN polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PTEN.
Immunogen
A synthetic peptide corresponding to C-terminus of human PTEN.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PTEN in PC-3 cell lysate with PTEN polyclonal antibody (Cat # PAB13148) at 1 ug/mL in either the (A) absence or (B) presence of blocking peptide. -
Gene Info — PTEN
Entrez GeneID
5728Protein Accession#
P60484Gene Name
PTEN
Gene Alias
10q23del, BZS, MGC11227, MHAM, MMAC1, PTEN1, TEP1
Gene Description
phosphatase and tensin homolog
Gene Ontology
HyperlinkGene Summary
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq
Other Designations
MMAC1 phosphatase and tensin homolog deleted on chromosome 10|OTTHUMP00000020032|mutated in multiple advanced cancers 1
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Interactome
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Pathway
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Disease
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Publication Reference
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The lipid phosphatase activity of PTEN is critical for its tumor supressor function.
Myers MP, Pass I, Batty IH, Van der Kaay J, Stolarov JP, Hemmings BA, Wigler MH, Downes CP, Tonks NK.
PNAS 1998 Nov; 95(23):13513.
Application:WB-Ce, WB-Tr, Human, U87MG, U373MG cells.
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Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers.
Steck PA, Pershouse MA, Jasser SA, Yung WK, Lin H, Ligon AH, Langford LA, Baumgard ML, Hattier T, Davis T, Frye C, Hu R, Swedlund B, Teng DH, Tavtigian SV.
Nature Genetics 1997 Apr; 15(4):356.
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PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer.
Li J, Yen C, Liaw D, Podsypanina K, Bose S, Wang SI, Puc J, Miliaresis C, Rodgers L, McCombie R, Bigner SH, Giovanella BC, Ittmann M, Tycko B, Hibshoosh H, Wigler MH, Parsons R.
Science 1997 Mar; 275(5308):1943.
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The lipid phosphatase activity of PTEN is critical for its tumor supressor function.
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