DIABLO polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of DIABLO in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide with DIABLO polyclonal antibody (Cat # PAB13089) at 1 ug /mL .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of DIABLO in human ovary tissue with DIABLO polyclonal antibody (Cat # PAB13089) at 5 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DIABLO.
Immunogen
A synthetic peptide corresponding to amino acids 225-239 of human DIABLO.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
human heart tissue lysate can be used as positive control and a 25 KDa band can be detected.
Form
Liquid
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of DIABLO in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide with DIABLO polyclonal antibody (Cat # PAB13089) at 1 ug /mL .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of DIABLO in human ovary tissue with DIABLO polyclonal antibody (Cat # PAB13089) at 5 ug/mL . -
Gene Info — DIABLO
Entrez GeneID
56616Protein Accession#
AAF87716Gene Name
DIABLO
Gene Alias
DIABLO-S, FLJ10537, FLJ25049, SMAC, SMAC3
Gene Description
diablo homolog (Drosophila)
Omim ID
605219Gene Ontology
HyperlinkGene Summary
This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. [provided by RefSeq
Other Designations
0610041G12Rik|diablo|direct IAP-binding protein with low pI|mitochondrial Smac protein|second mitochondria-derived activator of caspase
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Interactome
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Disease
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Publication Reference
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Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway.
Srinivasula SM, Datta P, Fan XJ, Fernandes-Alnemri T, Huang Z, Alnemri ES.
The Journal of Biological Chemistry 2000 Nov; 275(46):36152.
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Structural and biochemical basis of apoptotic activation by Smac/DIABLO.
Chai J, Du C, Wu JW, Kyin S, Wang X, Shi Y.
Nature 2000 Aug; 406(6798):855.
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Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition.
Du C, Fang M, Li Y, Li L, Wang X.
Cell 2000 Jul; 102(1):33.
Application：IF, WB-Ce, Human, HeLa cells.
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Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway.
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