Dffb polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Dffb in mouse lung (L) and kidney (K) tissue lysates with Dffb polyclonal antibody (Cat # PAB13074) at 1 : 500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Dffb in mouse kidney tissue with Dffb polyclonal antibody (Cat # PAB13074) at 1 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Dffb.
Immunogen
A synthetic peptide corresponding to amino acids 314-329 of mouse Dffb.
Host
Rabbit
Reactivity
Mouse
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Dffb in mouse lung (L) and kidney (K) tissue lysates with Dffb polyclonal antibody (Cat # PAB13074) at 1 : 500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Dffb in mouse kidney tissue with Dffb polyclonal antibody (Cat # PAB13074) at 1 ug/mL . -
Gene Info — Dffb
Entrez GeneID
13368Protein Accession#
O54788Gene Name
Dffb
Gene Alias
40kDa, 5730477D02Rik, CAD, CPAN, DFF40, Didff
Gene Description
DNA fragmentation factor, beta subunit
Gene Ontology
HyperlinkGene Summary
O
Other Designations
DNA fragmentation factor, 40 kD, beta subunit|DNase inhibited by DNA fragmentation factor|OTTMUSP00000011273|caspase-activated DNase
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Publication Reference
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The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis.
Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X.
PNAS 1998 Jul; 95(15):8461.
Application：WB-Tr, Human, HEK 293 cells.
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CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45.
Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT.
Current Biology 1998 Apr; 8(9):537.
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A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD.
Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S.
Nature 1998 Jan; 391(6662):43.
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The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis.
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