DFFA polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of DFFA in HeLa, (H), K-562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFFA polyclonal antibody (Cat # PAB13069) at 1 : 1000 dilution.
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of HeLa cells with 2 ug/mL DFFA polyclonal antibody (Cat # PAB13069).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DFFA.
Immunogen
A synthetic peptide corresponding to amino acids 2-21 of human DFFA.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
Western Blot (1:1000-1:2000)
Immunohistochemistry (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of DFFA in HeLa, (H), K-562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFFA polyclonal antibody (Cat # PAB13069) at 1 : 1000 dilution.Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of HeLa cells with 2 ug/mL DFFA polyclonal antibody (Cat # PAB13069). -
Gene Info — DFFA
Entrez GeneID
1676Protein Accession#
NP_004392Gene Name
DFFA
Gene Alias
DFF-45, DFF1, ICAD
Gene Description
DNA fragmentation factor, 45kDa, alpha polypeptide
Omim ID
601882Gene Ontology
HyperlinkGene Summary
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
DFF45|DNA fragmentation factor, 45 kD, alpha polypeptide|DNA fragmentation factor, 45 kD, alpha subunit|OTTHUMP00000001903|OTTHUMP00000001904
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Interactome
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Pathway
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Disease
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Publication Reference
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Functional interaction of DFF35 and DFF45 with caspase-activated DNA fragmentation nuclease DFF40.
Gu J, Dong RP, Zhang C, McLaughlin DF, Wu MX, Schlossman SF.
The Journal of Biological Chemistry 1999 Jul; 274(30):20759.
Application：WB-Tr, Human, HEK 293T cells.
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A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD.
Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S.
Nature 1998 Jan; 391(6662):43.
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DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis.
Liu X, Zou H, Slaughter C, Wang X.
Cell 1997 Apr; 89(2):175.
Application：WB-Ce, Human, U-937 cells.
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Functional interaction of DFF35 and DFF45 with caspase-activated DNA fragmentation nuclease DFF40.
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