MED4 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MED4.
Immunogen
A synthetic peptide corresponding to C-terminus 17 amino acids of human MED4.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of MED4 in human testis tissue lysate with MED4 polyclonal antibody (Cat # PAB13053) at 0.5 ug/mL .Immunohistochemistry
Immunohistochemical staining of human testis tissue with 10 ug/mL MED4 polyclonal antibody (Cat # PAB13053). -
Gene Info — MED4
Entrez GeneID
29079Protein Accession#
NP_054885Gene Name
MED4
Gene Alias
DRIP36, FLJ10956, HSPC126, RP11-90M2.2, TRAP36, VDRIP
Gene Description
mediator complex subunit 4
Omim ID
605718Gene Ontology
HyperlinkGene Summary
subunit 4 homolog|p36 TRAP/SMCC/PC2 subunit|vitamin D receptor interacting protein
Other Designations
OTTHUMP00000018390|mediator of RNA polymerase II transcription, subunit 4 homolog|p36 TRAP/SMCC/PC2 subunit|vitamin D receptor interacting protein
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Interactome
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Disease
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Publication Reference
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Identification of host proteins required for HIV infection through a functional genomic screen.
Brass AL, Dykxhoorn DM, Benita Y, Yan N, Engelman A, Xavier RJ, Lieberman J, Elledge SJ.
Science 2008 Jan; 319(5865):921.
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Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex.
Rachez C, Lemon BD, Suldan Z, Bromleigh V, Gamble M, Naar AM, Erdjument-Bromage H, Tempst P, Freedman LP.
Nature 1999 Apr; 398(6730):824.
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Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae.
Gustafsson CM, Myers LC, Beve J, Spahr H, Lui M, Erdjument-Bromage H, Tempst P, Kornberg RD.
The Journal of Biological Chemistry 1998 Nov; 273(47):30851.
Application:WB, Recombinant protein.
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Identification of host proteins required for HIV infection through a functional genomic screen.
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