BUB3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of BUB3 in Jurkat cell lysate with BUB3 polyclonal antibody (Cat # PAB12997) at (A) 0.5 and (B) 1 ug/mL .
Immunocytochemistry
Immunocytochemistry of BUB3 in Jurkat cells with BUB3 polyclonal antibody (Cat # PAB12997) at 10 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BUB3.
Immunogen
A synthetic peptide corresponding to C-terminus 16 amino acids of human BUB3.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Recommend Usage
Western Blot (0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of BUB3 in Jurkat cell lysate with BUB3 polyclonal antibody (Cat # PAB12997) at (A) 0.5 and (B) 1 ug/mL .Immunocytochemistry
Immunocytochemistry of BUB3 in Jurkat cells with BUB3 polyclonal antibody (Cat # PAB12997) at 10 ug/mL . -
Gene Info — BUB3
Entrez GeneID
9184Protein Accession#
O43684Gene Name
BUB3
Gene Alias
BUB3L, hBUB3
Gene Description
budding uninhibited by benzimidazoles 3 homolog (yeast)
Omim ID
603719Gene Ontology
HyperlinkGene Summary
This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
BUB3 budding uninhibited by benzimidazoles 3 homolog|OTTHUMP00000020679|OTTHUMP00000020680|budding uninhibited by benomyl|budding uninhibited by benzimidazoles 3|mitotic checkpoint component
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Interactome
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Pathway
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Disease
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Publication Reference
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Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis.
Kalitsis P, Earle E, Fowler KJ, Choo KH.
Genes & Development 2000 Sep; 14(18):2277.
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The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase.
Taylor SS, Ha E, McKeon F.
The Journal of Cell Biology 1998 Jul; 142(1):1.
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The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase.
Roberts BT, Farr KA, Hoyt MA.
Molecular and Cellular Biology 1994 Dec; 14(12):8282.
Application：WB-Ce, Yeast, Saccharomyces cerevisiae.
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Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis.
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