HUWE1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HUWE1.
Immunogen
A synthetic peptide corresponding to C-terminus 19 amino acids of human HUWE1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for up to one year.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HUWE1 in Daudi cell lysate with HUWE1 polyclonal antibody (Cat # PAB12996) at 1 ug/mL .Immunocytochemistry
Immunocytochemistry of HUWE1 in Daudi cells with HUWE1 polyclonal antibody (Cat # PAB12996) at 5 ug/mL . -
Gene Info — HUWE1
Entrez GeneID
10075Protein Accession#
NP_113584Gene Name
HUWE1
Gene Alias
ARF-BP1, HECTH9, HSPC272, Ib772, KIAA0312, LASU1, MULE, UREB1
Gene Description
HECT, UBA and WWE domain containing 1
Gene Ontology
HyperlinkGene Summary
O
Other Designations
ARF binding protein 1|BJ-HCC-24 tumor antigen|HECT domain protein LASU1|Mcl-1 ubiquitin ligase E3|OTTHUMP00000023356|OTTHUMP00000061860
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Interactome
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Pathway
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Disease
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Publication Reference
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TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation.
Ke Li, Feng Wang, Zhao-Na Yang, Ting-Ting Zhang, Yu-Fen Yuan, Chen-Xi Zhao, Zaiwuli Yeerjiang, Bing Cui, Fang Hua, Xiao-Xi Lv, Xiao-Wei Zhang, Jiao-Jiao Yu, Shan-Shan Liu, Jin-Mei Yu, Shuang Shang, Yang Xiao, Zhuo-Wei Hu.
Nature Communications 2020 Dec; 11(1):6316.
Application:WB-Tr, Human, Raji cells.
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The ARF tumour suppressor.
Gallagher SJ, Kefford RF, Rizos H.
The International Journal of Biochemistry & Cell Biology 2006 Feb; 38(10):1637.
Application:WB, Human, Mouse, Mammalian cells , MEFs.
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ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor.
Chen D, Kon N, Li M, Zhang W, Qin J, Gu W.
Cell 2005 Jul; 121(7):1071.
Application:WB, Human, HCT-116, NCI-H1299, U-2 OS cells.
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The Ink4a tumor suppressor gene product, p19Arf, interacts with MDM2 and neutralizes MDM2's inhibition of p53.
Pomerantz J, Schreiber-Agus N, Liegeois NJ, Silverman A, Alland L, Chin L, Potes J, Chen K, Orlow I, Lee HW, Cordon-Cardo C, DePinho RA.
Cell 1998 Mar; 92(6):713.
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TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation.
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