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Last updated: 2016/12/4
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CD274 polyclonal antibody

  • Catalog # : PAB12985
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CD274.
  • Immunogen:
  • A synthetic peptide corresponding to internal region 17 amino acids of human CD274.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • Has no cross-reactivity to CD273.
  • Form:
  • Liquid
  • Purification:
  • Peptide affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry (0.5 ug/mL)
    Immunofluorescence (20 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5 ug/mL)
    Western Blot (0.5-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C for three months. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of CD274 in Raji cell lysate with CD274 polyclonal antibody (Cat # PAB12985) at (A) 0.5 and (B) 1 ug/mL .
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of CD274 in human heart tissue with CD274 polyclonal antibody (Cat # PAB12985) at 2.5 ug/mL .
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of human heart cells with CD274 polyclonal antibody (Cat# PAB12985) under 20 ug/mL working concentration.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of A-20 cells with CD274 polyclonal antibody (Cat# PAB12985). Green: Isotype control. Red : CD274 antibody.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • CD274
  • Gene Alias:
  • B7-H,B7H1,MGC142294,MGC142296,PD-L1,PDCD1L1,PDCD1LG1,PDL1
  • Gene Description:
  • CD274 molecule
  • Other Designations:
  • CD274 antigen,OTTHUMP00000021029,programmed cell death 1 ligand 1
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