BMI1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of BMI1 in K-562 cell lysate with BMI1 polyclonal antibody (Cat # PAB12958) at (A) 0.5, (B) 1 and (C) 2 ug/mL .
Immunocytochemistry
Immunocytochemistry of BMI1 in K-562 cells with BMI1 polyclonal antibody (Cat # PAB12958) at 10 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BMI1.
Immunogen
A synthetic peptide corresponding to internal region 15 amino acids of human BMI1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of BMI1 in K-562 cell lysate with BMI1 polyclonal antibody (Cat # PAB12958) at (A) 0.5, (B) 1 and (C) 2 ug/mL .Immunocytochemistry
Immunocytochemistry of BMI1 in K-562 cells with BMI1 polyclonal antibody (Cat # PAB12958) at 10 ug/mL . -
Gene Info — BMI1
Entrez GeneID
648Protein Accession#
P35226Gene Name
BMI1
Gene Alias
MGC12685, PCGF4, RNF51
Gene Description
BMI1 polycomb ring finger oncogene
Omim ID
164831Gene Ontology
HyperlinkOther Designations
B lymphoma Mo-MLV insertion region 1 homolog|flvi-2/bmi-1|murine leukemia viral (bmi-1) oncogene homolog|oncogene BMI-1|polycomb group ring finger 4
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Interactome
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Publication Reference
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Bmi-1 determines the proliferative capacity of normal and leukaemic stem cells.
Lessard J, Sauvageau G.
Nature 2003 May; 423(6937):255.
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The oncogene and Polycomb-group gene bmi-1 regulates cell proliferation and senescence through the ink4a locus.
Jacobs JJ, Kieboom K, Marino S, DePinho RA, van Lohuizen M.
Nature 1999 Jan; 397(6715):164.
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Characterization and chromosomal localization of the human proto-oncogene BMI-1.
Alkema MJ, Wiegant J, Raap AK, Berns A, van Lohuizen M.
Human Molecular Genetics 1993 Oct; 2(10):1597.
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Bmi-1 determines the proliferative capacity of normal and leukaemic stem cells.
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