DAD1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 polyclonal antibody (Cat # PAB12919) at (A) 0.5, (B) 1, and (C) 2 ug/mL .
Immunocytochemistry
Immunocytochemistry of DAD1 in HepG2 cells with DAD1 polyclonal antibody (Cat # PAB12919) at 10 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DAD1.
Immunogen
A synthetic peptide corresponding to C-terminus 14 amino acids of human DAD1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Recommend Usage
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 polyclonal antibody (Cat # PAB12919) at (A) 0.5, (B) 1, and (C) 2 ug/mL .Immunocytochemistry
Immunocytochemistry of DAD1 in HepG2 cells with DAD1 polyclonal antibody (Cat # PAB12919) at 10 ug/mL . -
Gene Info — DAD1
Entrez GeneID
1603Protein Accession#
AAH09798Gene Name
DAD1
Gene Alias
OST2
Gene Description
defender against cell death 1
Omim ID
600243Gene Ontology
HyperlinkGene Summary
DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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DAD1 is required for the function and the structural integrity of the oligosaccharyltransferase complex.
Sanjay A, Fu J, Kreibich G.
The Journal of Biological Chemistry 1998 Oct; 273(40):26094.
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DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase.
Kelleher DJ, Gilmore R.
PNAS 1997 May; 94(10):4994.
Application：WB-Ce, WB-Re, Human, Recombinant protein, WI-38 cells.
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Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells.
Nakashima T, Sekiguchi T, Kuraoka A, Fukushima K, Shibata Y, Komiyama S, Nishimoto T.
Molecular and Cellular Biology 1993 Oct; 13(10):6367.
Application：WB-Ce, Human, Mouse, HeLa, tsBN7, BHK21 cells.
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DAD1 is required for the function and the structural integrity of the oligosaccharyltransferase complex.
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