Ripk3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Ripk3.
Immunogen
A synthetic peptide corresponding to amino acids 473-486 of mouse Ripk3.
Sequence
AQFGRGRGWQPFHK
Host
Rabbit
Reactivity
Mouse, Rat
Specificity
An approximately 57 KDa band can be detected.
Form
Liquid
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for up to one year.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Ripk3 in mouse NIH/3T3 whole cell lysate in the absence (A) or presence (B) of blocking peptide with Ripk3 polyclonal antibody (Cat # PAB12850) at 1 ug/mL .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Ripk3 in rat kidney tissue with Ripk3 polyclonal antibody (Cat # PAB12850) at 5 ug/mL . -
Gene Info — Ripk3
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Publication Reference
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Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-kappaB.
Pazdernik NJ, Donner DB, Goebl MG, Harrington MA.
Molecular and Cellular Biology 1999 Oct; 19(10):6500.
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RIP3, a novel apoptosis-inducing kinase.
Sun X, Lee J, Navas T, Baldwin DT, Stewart TA, Dixit VM.
The Journal of Biological Chemistry 1999 Jun; 274(24):16871.
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Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB.
Yu PW, Huang BC, Shen M, Quast J, Chan E, Xu X, Nolan GP, Payan DG, Luo Y.
Current Biology 1999 May; 9(10):539.
Application:IP, WB-Tr, Human, Phoenix-A cells.
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Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-kappaB.
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