RYBP polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of RYBP expression in human A-549 (lane A), HepG2 (lane B), and mouse NIH/3T3 (lane C) cell lysates with RYBP polyclonal antibody (Cat # PAB12843) at 1 ug /mL .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of RYBP in mouse liver tissue with RYBP polyclonal antibody (Cat # PAB12843) at 10 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RYBP.
Immunogen
A synthetic peptide corresponding to amino acids 215-228 of human RYBP.
Sequence
TPKGDMSAVNDESF
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
The sequence is identical to that of mouse origin. A band at approximately 32 KDa can be detected.
Form
Liquid
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for up to one year.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of RYBP expression in human A-549 (lane A), HepG2 (lane B), and mouse NIH/3T3 (lane C) cell lysates with RYBP polyclonal antibody (Cat # PAB12843) at 1 ug /mL .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of RYBP in mouse liver tissue with RYBP polyclonal antibody (Cat # PAB12843) at 10 ug/mL . -
Gene Info — RYBP
Entrez GeneID
23429Protein Accession#
AF179286Gene Name
RYBP
Gene Alias
AAP1, DEDAF, YEAF1
Gene Description
RING1 and YY1 binding protein
Omim ID
607535Gene Ontology
HyperlinkOther Designations
YY1 and E4TF1 associated factor 1|apoptin-associating protein 1|death effector domain-associated factor|ring1 interactor RYBP
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Interactome
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Disease
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Publication Reference
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The death effector domain-associated factor plays distinct regulatory roles in the nucleus and cytoplasm.
Zheng L, Schickling O, Peter ME, Lenardo MJ.
J Biol Chem 2001 Jun; 276(34):31945.
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RYBP, a new repressor protein that interacts with components of the mammalian Polycomb complex, and with the transcription factor YY1.
Garcia E, Marcos-Gutierrez C, del Mar Lorente M, Moreno JC, Vidal M.
The EMBO Journal 1999 Jun; 18(12):3404.
Application：IF, IP, WB-Tr, Human, HEK 293T, U-2 OS cells.
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The death effector domain-associated factor plays distinct regulatory roles in the nucleus and cytoplasm.
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