E2F7 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of E2F7.
Immunogen
A synthetic peptide corresponding to internal region of human E2F7.
Sequence
C-DLSKQKKFTPERNP
Host
Goat
Theoretical MW (kDa)
99.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot ( 0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
E2F7 polyclonal antibody (Cat # PAB12815) (0.03 ug/ml) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — E2F7
Entrez GeneID
144455Protein Accession#
NP_976328.2Gene Name
E2F7
Gene Alias
FLJ12981
Gene Description
E2F transcription factor 7
Gene Ontology
HyperlinkGene Summary
E2F transcription factors, such as E2F7, play an essential role in the regulation of cell cycle progression (Di Stefano et al., 2003 [PubMed 14633988]).[supplied by OMIM
Other Designations
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Interactome
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Disease
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Publication Reference
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Synergistic function of E2F7 and E2F8 is essential for cell survival and embryonic development.
Li J, Ran C, Li E, Gordon F, Comstock G, Siddiqui H, Cleghorn W, Chen HZ, Kornacker K, Liu CG, Pandit SK, Khanizadeh M, Weinstein M, Leone G, de Bruin A.
Developmental Cell 2008 Jan; 14(1):62.
Application:WB, Human, HeLa cells.
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Synergistic function of E2F7 and E2F8 is essential for cell survival and embryonic development.
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