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Last updated: 2017/2/19

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HMGB3 polyclonal antibody

  • Catalog # : PAB12814
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of HMGB3.
  • Immunogen:
  • A synthetic peptide corresponding to human HMGB3.
  • Sequence:
  • C-KFDGAKGPAKVARKK
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 23
  • Reactivity:
  • Human
  • Specificity:
  • 24 KDa band observed in human Lung lysates (calculated MW of 23 KDa according to NP_005333.2).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:64000)
    Western blot (0.01-0.03 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • HMGB3 polyclonal antibody (Cat # PAB12814) (0.01 ug/mL) staining of human lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3149
  • Gene Name:
  • HMGB3
  • Gene Alias:
  • HMG2A,HMG4,MGC90319
  • Gene Description:
  • high-mobility group box 3
  • Gene Summary:
  • HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000025865,high-mobility group (nonhistone chromosomal) protein 4,non-histone chromosomal protein
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