HMGB3 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
HMGB3 polyclonal antibody (Cat # PAB12814) (0.01 ug/mL) staining of human lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of HMGB3.
Immunogen
A synthetic peptide corresponding to human HMGB3.
Sequence
C-KFDGAKGPAKVARKK
Host
Goat
Theoretical MW (kDa)
23
Reactivity
Human
Specificity
24 KDa band observed in human Lung lysates (calculated MW of 23 KDa according to NP_005333.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
HMGB3 polyclonal antibody (Cat # PAB12814) (0.01 ug/mL) staining of human lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HMGB3
Entrez GeneID
3149Protein Accession#
NP_005333.2Gene Name
HMGB3
Gene Alias
HMG2A, HMG4, MGC90319
Gene Description
high-mobility group box 3
Omim ID
300193Gene Ontology
HyperlinkGene Summary
HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).[supplied by OMIM
Other Designations
OTTHUMP00000025865|high-mobility group (nonhistone chromosomal) protein 4|non-histone chromosomal protein
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Interactome
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Publication Reference
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let-7 regulates self renewal and tumorigenicity of breast cancer cells.
Yu F, Yao H, Zhu P, Zhang X, Pan Q, Gong C, Huang Y, Hu X, Su F, Lieberman J, Song E.
Cell 2007 Dec; 131(6):1109.
Application：WB, Human, SK-3rd, SKBR3 cells.
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let-7 regulates self renewal and tumorigenicity of breast cancer cells.
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