STAT5A polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The tissue lysate derived from mouse liver was immunoblotted by STAT5A polyclonal antibody (Cat # PAB12786) at 1 : 500.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast carcinoma tissue stained with STAT5A polyclonal antibody (Cat # PAB12786) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of STAT5A.
Immunogen
A synthetic peptide corresponding to residues surrounding Y694 (DGYVK) of human, mouse and rat STAT5A.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes STAT5A without a phosphorylated sites of Tyr694. It does not cross-react with phosphospecific peptide.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
The tissue lysate derived from mouse liver was immunoblotted by STAT5A polyclonal antibody (Cat # PAB12786) at 1 : 500.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast carcinoma tissue stained with STAT5A polyclonal antibody (Cat # PAB12786) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — STAT5A
Entrez GeneID
6776Gene Name
STAT5A
Gene Alias
MGF, STAT5
Gene Description
signal transducer and activator of transcription 5A
Omim ID
601511Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq
Other Designations
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Gene Info — Stat5a
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Gene Info — Stat5a
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Interactome
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Pathway
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Disease
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Publication Reference
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Cloning of human Stat5B. Reconstitution of interleukin-2-induced Stat5A and Stat5B DNA binding activity in COS-7 cells.
Lin JX, Mietz J, Modi WS, John S, Leonard WJ.
The Journal of Biological Chemistry 1996 May; 271(18):10738.
Application：IP, IP-WB, WB-Ce, Rabbit, Reticulocyte.
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Cloning of human Stat5B. Reconstitution of interleukin-2-induced Stat5A and Stat5B DNA binding activity in COS-7 cells.
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