STAT3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The whole cell lysate derived from A-431 was immunoblotted by STAT3 polyclonal antibody (Cat # PAB12784) at 1 : 500.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : STAT3 (pS727) (Phosphospecific)
B : STAT3 (Paired S727) (Nonphosphospeicfic)
were blotted at a 1 : 2000 dilution by :
1 : STAT3 polyclonal antibody (Cat # PAB12784),
2 : Phospho-STAT3 S727 polyclonal antibody (Cat # PAB12753).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of STAT3.
Immunogen
A synthetic peptide corresponding to residues surrounding S727 (PMSPR) of human, mouse, rat, bovine, chicken and dog STAT3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes STAT3 without a phosphorylated sites of Ser 727.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The whole cell lysate derived from A-431 was immunoblotted by STAT3 polyclonal antibody (Cat # PAB12784) at 1 : 500.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : STAT3 (pS727) (Phosphospecific)
B : STAT3 (Paired S727) (Nonphosphospeicfic)
were blotted at a 1 : 2000 dilution by :
1 : STAT3 polyclonal antibody (Cat # PAB12784),
2 : Phospho-STAT3 S727 polyclonal antibody (Cat # PAB12753). -
Gene Info — STAT3
Entrez GeneID
6774Gene Name
STAT3
Gene Alias
APRF, FLJ20882, HIES, MGC16063
Gene Description
signal transducer and activator of transcription 3 (acute-phase response factor)
Omim ID
102582Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
DNA-binding protein APRF|acute-phase response factor|signal transducer and activator of transcription 3
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Gene Info — Stat3
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Gene Info — Stat3
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Gene Info — STAT3
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Gene Info — STAT3
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Gene Info — STAT3
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Interactome
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Pathway
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Disease
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Publication Reference
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A new expression cloning strategy for isolation of substrate-specific kinases by using phosphorylation site-specific antibody.
Matsuo R, Ochiai W, Nakashima K, Taga T.
Journal of Immunology 2001 Jan; 247(1-2):141.
Application：WB-Ce, WB-Tr, Bacteria, Monkey, BAFh130, COS-7 cells.
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A new expression cloning strategy for isolation of substrate-specific kinases by using phosphorylation site-specific antibody.
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