SOCS3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The tissue lysates derived from mouse liver (Lane 1), mouse muscle (Lane 2) were immunoprobed by SOCS3 polyclonal antibody (Cat # PAB12780) at 1 : 500. An immunoreactive band is observed around ~27kDa.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SOCS3.
Immunogen
A synthetic peptide corresponding to C-terminus of human, mouse and rat SOCS3.
Host
Rabbit
Theoretical MW (kDa)
27
Reactivity
Human, Mouse
Specificity
This antibody recognizes ~27 KDa of SOCS3.
Form
Liquid
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
The tissue lysates derived from mouse liver (Lane 1), mouse muscle (Lane 2) were immunoprobed by SOCS3 polyclonal antibody (Cat # PAB12780) at 1 : 500. An immunoreactive band is observed around ~27kDa.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — SOCS3
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Gene Info — Socs3
Entrez GeneID
12702Gene Name
Socs3
Gene Alias
CIS3, Cish3, EF-10, SOCS-3, SSI-3
Gene Description
suppressor of cytokine signaling 3
Gene Ontology
HyperlinkOther Designations
E2a-Pbx1 target gene in fibroblasts 10|OTTMUSP00000003963|STAT-induced STAT inhibitor 3|cytokine inducible SH2-containing protein 3|cytokine-inducible SH2 protein 3
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Gene Info — Socs3
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Interactome
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Pathway
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Disease
- Anorexia Nervosa
- Arthritis
- Asthma
- Atherosclerosis
- Birth Weight
- Body Weight
- Bronchiolitis
- Bulimia
- Diabetes Mellitus
+ View More Disease
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Publication Reference
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SOCS3 targets Siglec 7 for proteasomal degradation and blocks Siglec 7-mediated responses.
Orr SJ, Morgan NM, Buick RJ, Boyd CR, Elliott J, Burrows JF, Jefferies CA, Crocker PR, Johnston JA.
The Journal of Biological Chemistry 2007 Feb; 282(6):3418.
Application：IP, WB, Mouse, Ba/F3 cells, MEFs.
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SOCS3 targets Siglec 7 for proteasomal degradation and blocks Siglec 7-mediated responses.
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