Shaker polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Shaker.
Immunogen
A synthetic peptide corresponding to N-terminus of Shaker.
Host
Rabbit
Theoretical MW (kDa)
73
Reactivity
Fruit fly, Human
Specificity
This antibody recognizes ~73 KDa of Shaker protein.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Essential role for dlg in synaptic clustering of Shaker K+ channels in vivo.
Tejedor FJ, Bokhari A, Rogero O, Gorczyca M, Zhang J, Kim E, Sheng M, Budnik V.
Journal of Neuroscience 1997 Jan; 17(1):152.
Application：IF, WB-Ti, Firefly, Monkey, COS-7 cells, Drosophila larva.
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Essential role for dlg in synaptic clustering of Shaker K+ channels in vivo.
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