LCK polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The whole cell lysate derived from Jurkat was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by LCK polyclonal antibody (Cat # PAB12774) at 1 : 500. An immunoreactive band around ~56 kDa is observed.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LCK.
Immunogen
A synthetic peptide corresponding to C-terminus of human, rat, bovine and dog LCK.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes LCK.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The whole cell lysate derived from Jurkat was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by LCK polyclonal antibody (Cat # PAB12774) at 1 : 500. An immunoreactive band around ~56 kDa is observed.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — LCK
Entrez GeneID
3932Gene Name
LCK
Gene Alias
YT16, p56lck, pp58lck
Gene Description
lymphocyte-specific protein tyrosine kinase
Omim ID
153390Gene Ontology
HyperlinkGene Summary
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq
Other Designations
T-lymphocyte specific protein tyrosine kinase p56lck|p56(LSTRA) protein-tyrosine kinase|protein tyrosine kinase|proto-oncogene tyrosine-protein kinase LCK
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Gene Info — Lck
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Gene Info — LCK
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Gene Info — LCK
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Interactome
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Pathway
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Disease
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Publication Reference
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Suppression of proximal T cell receptor signaling and lytic function in CD8+ tumor-infiltrating T cells.
Monu N, Frey AB.
Cancer Research 2007 Dec; 67(23):11447.
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Suppression of proximal T cell receptor signaling and lytic function in CD8+ tumor-infiltrating T cells.
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