POU2F1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of POU2F1.
Immunogen
A synthetic peptide corresponding to C-terminus of human, mouse and rat POU2F1.
Host
Rabbit
Theoretical MW (kDa)
89
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~89 KDa of POU2F1. It does not cross react with POU2F2, POU5F1.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
The tissue lysate derived from mouse kidney was immunoprobed by POU2F1 polyclonal antibody (Cat # PAB12771) at 1 : 500.
An immunoreactive band is observed around ~89 kDa (1).
This band is abolished by pre-incubation with immunizing peptide (2).Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptides was blotted onto NC membrane.
A : POU2F1
B : POU2F2
C : POU5F1
Followed by immunoblotting at a 1 : 1000 dilution by :
1 : POU2F1 polyclonal antibody (Cat # PAB12771)
2 : POU2F2 polyclonal antibody (Cat # PAB12772)
3 : POU5F1 polyclonal antibody (Cat # PAB12773) -
Gene Info — POU2F1
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Gene Info — Pou2f1
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Gene Info — Pou2f1
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Interactome
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Disease
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Publication Reference
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The POU transcription factor Oct-1 represses virus-induced interferon A gene expression.
Mesplede T, Island ML, Christeff N, Petek F, Doly J, Navarro S.
Molecular and Cellular Biochemistry 2005 Oct; 25(19):8717.
Application:Func, IP, Mouse, L929 cells.
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The POU transcription factor Oct-1 represses virus-induced interferon A gene expression.
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