GFP polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
The cell lysate of 293T with over-expressed recombinant protein GFP was resolved onto 10% SDS-PAGE, then transferred onto NC membrane. Followed by an immune-blotting with GFP polyclonal antibody (Cat # PAB12768) at 1 : 1000.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant GFP.
Immunogen
Recombinant protein corresponding to full length GFP.
Host
Rabbit
Theoretical MW (kDa)
26
Specificity
This antibody recognizes ~ 26 KDa of GFP.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Transfected lysate)
The cell lysate of 293T with over-expressed recombinant protein GFP was resolved onto 10% SDS-PAGE, then transferred onto NC membrane. Followed by an immune-blotting with GFP polyclonal antibody (Cat # PAB12768) at 1 : 1000.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Chemical and physical properties of aequorin and the green fluorescent protein isolated from Aequorea forskalea.
Prendergast FG, Mann KG.
Biochemistry 1978 Aug; 17(17):3448.
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Chemical and physical properties of aequorin and the green fluorescent protein isolated from Aequorea forskalea.
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