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Last updated: 2016/12/4
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DYKDDDDK tag polyclonal antibody

  • Catalog # : PAB12767
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptides of DYKDDDDK tag.
  • Immunogen:
  • A synthetic peptide corresponding to DYKDDDDK tag.
  • Host:
  • Rabbit
  • Specificity:
  • This antibody recognizes FLAG tagged fusion proteins.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • The whole cell lysate from the over-expressed FLAG tagged recombinant protein was resolved onto 10% SDS-PAGE, then transferred onto NC membrane. Followed by an immune-blotting with DYKDDDDK tag polyclonal antibody (Cat # PAB12767) at 1 : 1000. Lane 2 was pre-incubated by immunizing peptide.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
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