DYKDDDDK tag polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptides of DYKDDDDK tag.
Immunogen
A synthetic peptide corresponding to DYKDDDDK tag.
Host
Rabbit
Specificity
This antibody recognizes FLAG tagged fusion proteins.
Form
Liquid
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Transfected lysate)
The whole cell lysate from the over-expressed FLAG tagged recombinant protein was resolved onto 10% SDS-PAGE, then transferred onto NC membrane. Followed by an immune-blotting with DYKDDDDK tag polyclonal antibody (Cat # PAB12767) at 1 : 1000. Lane 2 was pre-incubated by immunizing peptide.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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