E tag polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
The whole cell lysate of NIH/ 3T3 with over-expressed E-Tag fused recombinant protein was resolved onto 10% SDS-PAGE, then transferred onto NC membrane. Followed by an immune-blotting with E tag polyclonal antibody (Cat # PAB12765) at 1 : 1000. Lane 2 was pre-incubated by immunizing peptide.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptides of E tag.
Immunogen
A synthetic peptide corresponding to E tag.
Sequence
GAPVPYPDPLEPR
Host
Rabbit
Specificity
This antibody recognizes E-tagged fusion proteins.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Transfected lysate)
The whole cell lysate of NIH/ 3T3 with over-expressed E-Tag fused recombinant protein was resolved onto 10% SDS-PAGE, then transferred onto NC membrane. Followed by an immune-blotting with E tag polyclonal antibody (Cat # PAB12765) at 1 : 1000. Lane 2 was pre-incubated by immunizing peptide.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane.
Campbell AM, Kessler PD, Fambrough DM.
The Journal of Biological Chemistry 1992 May; 267(13):9321.
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The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane.
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