GJA1 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human heart tissue with GJA1 polyclonal antibody (Cat # PAB12759) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by colling at RT for 20 min.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GJA1.
Immunogen
A synthetic peptide corresponding to segment of the 3rd cytoplasmic domain of human/rat GJA1.
Host
Rabbit
Theoretical MW (kDa)
43
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes 43 kDa of Connexin 43 derived from heart tissue or A549 cell lysate by Western blot. It reacts with human, rat and mouse. The other species are not tested.
Form
Liquid
Purification
Epitope affinity purification
Recommend Usage
ELISA (0.01-0.1 ug/mL)
Immunohistochemistry
Immunoprecipitation (2-5 ug/mL)
Western Blot (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (Antibody Stabilizer).
Storage Instruction
Store at 4°C for one month without detectable loss of activity. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. Upon initial thawing, apportion into working aliquots and store at -80°C for up to six months. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human heart tissue with GJA1 polyclonal antibody (Cat # PAB12759) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by colling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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