Rabbit polyclonal antibody raised against synthetic peptide of GJA1.
A synthetic peptide corresponding to the 3rd cytoplasmic domain of human and rat GJA1.
Theoretical MW (kDa):
Human, Mouse, Rat
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) The optimal working dilution should be determined by the end user.
In TBS, pH 7.2 (BSA, 10% Proclin300)
Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. Upon initial thawing, apportion into working aliquots and store at -80°C for up to six months.
Immunohistochemical staining of human heart tissue with GJA1 polyclonal antibody (Cat # PAB12759) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by colling at RT for 20 min.