CISH polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The tissue lysate derived from mouse kidney was immunoprobed by CISH polyclonal antibody (Cat # PAB12758) at 1 : 500. An immunoreactive band is observed around ~ 28kDa.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CISH.
Immunogen
A synthetic peptide corresponding to internal domain of human, mouse and rat CISH.
Host
Rabbit
Theoretical MW (kDa)
29
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
The tissue lysate derived from mouse kidney was immunoprobed by CISH polyclonal antibody (Cat # PAB12758) at 1 : 500. An immunoreactive band is observed around ~ 28kDa.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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CIS associates with the interleukin-2 receptor beta chain and inhibits interleukin-2-dependent signaling.
Aman MJ, Migone TS, Sasaki A, Ascherman DP, Zhu M, Soldaini E, Imada K, Miyajima A, Yoshimura A, Leonard WJ.
The Journal of Biological Chemistry 1999 Oct; 274(42):30266.
Application：IP, WB-Ce, WB-Tr, Human, Mouse, Ba/F3, HEK 293T cells, Peripheral blood lymphocytes.
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CIS associates with the interleukin-2 receptor beta chain and inhibits interleukin-2-dependent signaling.
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