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Last updated: 2016/12/4
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STAT5A (phospho Y694) polyclonal antibody

  • Catalog # : PAB12756
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of STAT5A.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding Y694 of human, mouse and rat STAT5A.
  • Sequence:
  • DGYVK
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody recognizes STAT5 with a phosphorylated sites of Tyr694. It does not cross-react with non-phosphospecific peptide.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (1:100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • The tissue lysate derived from GH stimulated mouse liver was immunoblotted by STAT5A (phospho Y694) polyclonal antibody (Cat # PAB12756) at 1 : 500 (Lane 1). Lane 2 is a negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human breast cancer tissue stained with STAT5A (phospho Y694) polyclonal antibody (Cat # PAB12756) at 1 : 100 for 10 min at RT.
    Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
  • ELISA
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