STAT4 (phospho Y693) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The whole tissue lysate derived from mouse kidney was immunoblotted by STAT4 (phospho Y693) polyclonal antibody (Cat # PAB12755) at 1 : 500.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : STAT4 (pY694, Phospho-specific).
B : STAT4 (Paired Y694, Non phospho-speicfic).
C : Non-related phospho-specific peptide were blotted at a 1 : 2000 dilution by STAT4 (phospho Y693) polyclonal antibody (Cat # PAB12755).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of STAT4.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y693 of human, mouse, rat, bovine, chicken and dog STAT4.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody recognizes STAT4 with a phosphorylated sites of Tyr693. It does not cross-react with non-phosphospecific peptide.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
The whole tissue lysate derived from mouse kidney was immunoblotted by STAT4 (phospho Y693) polyclonal antibody (Cat # PAB12755) at 1 : 500.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : STAT4 (pY694, Phospho-specific).
B : STAT4 (Paired Y694, Non phospho-speicfic).
C : Non-related phospho-specific peptide were blotted at a 1 : 2000 dilution by STAT4 (phospho Y693) polyclonal antibody (Cat # PAB12755). -
Publication Reference
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High basal STAT4 balanced by STAT1 induction to control type 1 interferon effects in natural killer cells.
Miyagi T, Gil MP, Wang X, Louten J, Chu WM, Biron CA.
The Journal of Experimental Medicine 2007 Oct; 204(10):2383.
Application：Flow Cyt, WB, Mouse, Mouse natural killer cells, splenic cells.
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High basal STAT4 balanced by STAT1 induction to control type 1 interferon effects in natural killer cells.
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