STAT3 (phospho S727) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The whole cell lysate derived from A-431 was immunoblotted by STAT3 (phospho S727) polyclonal antibody (Cat # PAB12753) at 1 : 500.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : STAT3 (pS727 , Phosphospecific).
B : STAT3 (Paired S727, Non phospho-speicfic).
Followed were blotted at a 1 : 2000 dilution by :
1 : STAT3 polyclonal antibody (Cat # PAB12784).
2 : STAT3 (phospho S727) polyclonal antibody (Cat # PAB12753).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of STAT3.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S727 of human, mouse, rat, bovine, chicken and dog STAT3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes STAT3 with a phosphorylated sites of Ser 727. It does not cross-react with non-phosphospecific peptide.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The whole cell lysate derived from A-431 was immunoblotted by STAT3 (phospho S727) polyclonal antibody (Cat # PAB12753) at 1 : 500.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : STAT3 (pS727 , Phosphospecific).
B : STAT3 (Paired S727, Non phospho-speicfic).
Followed were blotted at a 1 : 2000 dilution by :
1 : STAT3 polyclonal antibody (Cat # PAB12784).
2 : STAT3 (phospho S727) polyclonal antibody (Cat # PAB12753). -
Publication Reference
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A new expression cloning strategy for isolation of substrate-specific kinases by using phosphorylation site-specific antibody.
Matsuo R, Ochiai W, Nakashima K, Taga T.
Journal of Immunology 2001 Jan; 247(1-2):141.
Application：ELISA, IP, WB-Ce, WB-Tr, Bacteria, Monkey, BAFh130, COS-7 cells.
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A new expression cloning strategy for isolation of substrate-specific kinases by using phosphorylation site-specific antibody.
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