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Last updated: 2016/12/4
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STAT3 (phospho S727) polyclonal antibody

  • Catalog # : PAB12753
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of STAT3.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S727 of human, mouse, rat, bovine, chicken and dog STAT3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody recognizes STAT3 with a phosphorylated sites of Ser 727. It does not cross-react with non-phosphospecific peptide.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (1:50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The whole cell lysate derived from A-431 was immunoblotted by STAT3 (phospho S727) polyclonal antibody (Cat # PAB12753) at 1 : 500.
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot Blot : 1 ug peptide was blot onto NC membrane.
    A : STAT3 (pS727 , Phosphospecific).
    B : STAT3 (Paired S727, Non phospho-speicfic).
    Followed were blotted at a 1 : 2000 dilution by :
    1 : STAT3 polyclonal antibody (Cat # PAB12784).
    2 : STAT3 (phospho S727) polyclonal antibody (Cat # PAB12753).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
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