STAT1 (phospho Y701) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The tissue lysate derived from GH stimulated mouse liver was immunoblotted by STAT1 (phospho Y701) polyclonal antibody (Cat # PAB12752) at 1 : 500.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : STAT1 (pY701, Phospho-specific).
B : STAT1 (Paired Y701, Non phospho-speicfic).
Followed were blotted at a 1 : 2000 dilution by :
1 : STAT1 polyclonal antibody (Cat # PAB12783).
2 : STAT1 (phospho Y701) polyclonal antibody (Cat # PAB12752).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of STAT1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y701 of human, mouse and rat STAT1.
Sequence
TGYIK
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes STAT1 with a phosphorylated sites of Tyr701. It does not cross-react with non-phosphospecific peptide.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
The tissue lysate derived from GH stimulated mouse liver was immunoblotted by STAT1 (phospho Y701) polyclonal antibody (Cat # PAB12752) at 1 : 500.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : STAT1 (pY701, Phospho-specific).
B : STAT1 (Paired Y701, Non phospho-speicfic).
Followed were blotted at a 1 : 2000 dilution by :
1 : STAT1 polyclonal antibody (Cat # PAB12783).
2 : STAT1 (phospho Y701) polyclonal antibody (Cat # PAB12752). -
Publication Reference
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SHP-2 is a dual-specificity phosphatase involved in Stat1 dephosphorylation at both tyrosine and serine residues in nuclei.
Wu TR, Hong YK, Wang XD, Ling MY, Dragoi AM, Chung AS, Campbell AG, Han ZY, Feng GS, Chin YE.
The Journal of Biological Chemistry 2002 Dec; 277(49):47572.
Application：WB-Ce, WB-Tr, Human, Mouse, A-431 cells, HEK 293T cells, MEFs.
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SHP-2 is a dual-specificity phosphatase involved in Stat1 dephosphorylation at both tyrosine and serine residues in nuclei.
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