PTEN (phospho S385) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with PTEN (phospho S385) polyclonal antibody (Cat # PAB12751, lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (lane 2).
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : PTEN (pS385, Phospho-specific).
B : PTEN (Paired S385, Non-phospho-speicfic).
C : Non-related phospho-specific peptide.
Followed were blotted at a 1 : 2000 dilution by :
1. PTEN (phospho S385) polyclonal antibody (Cat # PAB12751).
2. PTEN polyclonal antibody (Cat # PAB12725).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PTEN.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S385 of human, mouse and rat PTEN.
Sequence
DTSDP
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes PTEN with a phosphorylated site at Serine 385. It does not cross-react with non-phosphospecific peptide.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with PTEN (phospho S385) polyclonal antibody (Cat # PAB12751, lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (lane 2).Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : PTEN (pS385, Phospho-specific).
B : PTEN (Paired S385, Non-phospho-speicfic).
C : Non-related phospho-specific peptide.
Followed were blotted at a 1 : 2000 dilution by :
1. PTEN (phospho S385) polyclonal antibody (Cat # PAB12751).
2. PTEN polyclonal antibody (Cat # PAB12725). -
Publication Reference
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Cooperative phosphorylation of the tumor suppressor phosphatase and tensin homologue (PTEN) by casein kinases and glycogen synthase kinase 3beta.
Al-Khouri AM, Ma Y, Togo SH, Williams S, Mustelin T.
The Journal of Biological Chemistry 2005 Aug; 280(42):35195.
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Cooperative phosphorylation of the tumor suppressor phosphatase and tensin homologue (PTEN) by casein kinases and glycogen synthase kinase 3beta.
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