The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with PTEN (phospho S385) polyclonal antibody (Cat # PAB12751, lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (lane 2).
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane. A : PTEN (pS385, Phospho-specific). B : PTEN (Paired S385, Non-phospho-speicfic). C : Non-related phospho-specific peptide. Followed were blotted at a 1 : 2000 dilution by : 1. PTEN (phospho S385) polyclonal antibody (Cat # PAB12751). 2. PTEN polyclonal antibody (Cat # PAB12725).