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Last updated: 2016/11/27
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PTEN (phospho S385) polyclonal antibody

  • Catalog # : PAB12751
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of PTEN.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S385 of human, mouse and rat PTEN.
  • Sequence:
  • DTSDP
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody recognizes PTEN with a phosphorylated site at Serine 385. It does not cross-react with non-phosphospecific peptide.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (1:200)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with PTEN (phospho S385) polyclonal antibody (Cat # PAB12751, lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (lane 2).
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot Blot : 1 ug peptide was blot onto NC membrane.
    A : PTEN (pS385, Phospho-specific).
    B : PTEN (Paired S385, Non-phospho-speicfic).
    C : Non-related phospho-specific peptide.
    Followed were blotted at a 1 : 2000 dilution by :
    1. PTEN (phospho S385) polyclonal antibody (Cat # PAB12751).
    2. PTEN polyclonal antibody (Cat # PAB12725).
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
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