PRKAA1 (phospho S79) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The whole cell lysate derived from serum starved HEK 293 was immunoblotted by Prkaa1 (phopho S79) polyclonal antibody (Cat # PAB12747).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PRKAA1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S79 of Carassius auratus PRKAA1.
Sequence
CGSPN
Host
Rabbit
Reactivity
Human
Specificity
This antibody recognizes PRKAA1 with a phosphorylated site of Ser 79. It does not cross-react with non-phosphospecific peptide.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The whole cell lysate derived from serum starved HEK 293 was immunoblotted by Prkaa1 (phopho S79) polyclonal antibody (Cat # PAB12747).Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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AMPK regulation of mouse oocyte meiotic resumption in vitro.
Chen J, Hudson E, Chi MM, Chang AS, Moley KH, Hardie DG, Downs SM.
Developmental Biology 2006 Mar; 291(2):227.
Application：WB-Ce, Mouse, Mouse Oocyte.
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AMPK regulation of mouse oocyte meiotic resumption in vitro.
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