Trimethyl-Histone H3 polyclonal antibody
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More Files
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Immunofluorescence
Fluorescent Immunohistochemistry : NIH/3T3 cells were cultured in chamber, and fixed onto slide followed by incubation with 5 ug/mL Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12742), then Cy3 labeled Gt anti Rb IgG for visualization under fluorescent microscope.
Immunoprecipitation
The nuclear extract derived from NIH/3T3 were immunoprecipitated by 4 ug of Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12742), then probed with Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12742) at 1 : 1000.
An immunoreactive band at ~17 KDa is observed.
Note : ~50 KDa and ~22 KDa are the rabbit immunoglobulin heavy and light chains.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Trimethyl-Histone H3.
Immunogen
A synthetic peptide corresponding to trimethyl lysine 9 of human Histone H3.
Host
Rabbit
Theoretical MW (kDa)
17
Reactivity
Bovine, Chicken, Human, Mouse, Rat
Specificity
This antibody only recognizes ~ 17 KDa of Trimethylated Histone H3 at K9. It does not cross react to non-methylated H3 or mono-, di-methylated Histone H3.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunofluorescence
Fluorescent Immunohistochemistry : NIH/3T3 cells were cultured in chamber, and fixed onto slide followed by incubation with 5 ug/mL Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12742), then Cy3 labeled Gt anti Rb IgG for visualization under fluorescent microscope.Immunoprecipitation
The nuclear extract derived from NIH/3T3 were immunoprecipitated by 4 ug of Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12742), then probed with Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12742) at 1 : 1000.
An immunoreactive band at ~17 KDa is observed.
Note : ~50 KDa and ~22 KDa are the rabbit immunoglobulin heavy and light chains.Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins.
Seward DJ, Cubberley G, Kim S, Schonewald M, Zhang L, Tripet B, Bentley DL.
Nature Structural & Molecular Biology 2007 Feb; 14(3):240.
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Histone H3 lysine 9 methylation and HP1gamma are associated with transcription elongation through mammalian chromatin.
Vakoc CR, Mandat SA, Olenchock BA, Blobel GA.
Molecular Cell 2005 Aug; 19(3):381.
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Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins.
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