Product Browser

Last updated: 2017/9/17
  • Related Product Showcase
  • By Application

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

Trimethyl-Histone H3 polyclonal antibody

  • Catalog # : PAB12741
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Trimethyl-Histone H3.
  • Immunogen:
  • A synthetic peptide corresponding to trimethyl lysine 4 of human Histone H3.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 17
  • Reactivity:
  • Bovine, Chicken, Human, Mouse, Rat
  • Specificity:
  • This antibody only recognizes ~ 17 KDa of Trimethylated Histone H3 at K4. It does not cross react to non-methylated H3 or mono-, di-methylated Histone H3.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (1:100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Fluorescent Immunohistochemistry : NIH/3T3 cells were cultured in chamber, and fixed onto slide followed by incubation with Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12741) at 1 : 100 for 10 min at RT. FITC labeled Gt anti Rb IgG was used for visualization under fluorescent microscope.
  • Immunoprecipitation
  • Immunoprecipitation
  • The nuclear extract derived from NIH/3T3 were immunoprecipitated by 4 ug of Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12741) (Lane 2) and a rabbit IgG as a control (Lane 1), then probed with Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12741) at 1 : 1000.
    An immunoreactive band at ~17 KDa is observed.
    Note: ~50 KDa and ~22 KDa are the rabbit immunoglobulin heavy and light chain.
  • ELISA
  • Application Image
  • Western Blot
  • Immunohistochemistry
  • ELISA
  • RSS
  • YouTube
  • Linkedin
  • Facebook