Trimethyl-Histone H3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Trimethyl-Histone H3.
Immunogen
A synthetic peptide corresponding to trimethyl lysine 27 of human Histone H3.
Host
Rabbit
Theoretical MW (kDa)
17
Reactivity
Bovine, Chicken, Human, Mouse, Rat
Specificity
This antibody only recognizes ~ 17 KDa of Trimethylated Histone H3 at K27. It does not cross react to non-methylated H3 or mono-, di-methylated Histone H3.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunofluorescence
Fluorescent Immunohistochemistry : NIH/3T3 cells were cultured in chamber, and fixed onto slide followed by incubation with 5 ug/mL Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12740), then Cy3 labeled Gt anti Rb IgG for visualization under fluorescent microscope.Immunoprecipitation
The nuclear extract derived from NIH/3T3 were immunoprecipitated by 4 ug of Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12740), then probed with Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12740) at 1 : 1000. An immunoreactive band at ~17 KDa is observed.
Note : ~50 KDa and ~22 KDa are the rabbit immunoglobulin heavy and light chain.Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Long noncoding RNA NEAT1 suppresses hepatocyte proliferation in fulminant hepatic failure through increased recruitment of EZH2 to the LATS2 promoter region and promotion of H3K27me3 methylation.
Qiang Wang, Lian Liu, Sheng Zhang, Yingzi Ming, Shu Liu, Ke Cheng, Yujun Zhao.
Experimental & Molecular Medicine 2020 Mar; 52(3):461.
Application:ChIP, Human, HL-7702 cells.
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Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins.
Seward DJ, Cubberley G, Kim S, Schonewald M, Zhang L, Tripet B, Bentley DL.
Nature Structural & Molecular Biology 2007 Feb; 14(3):240.
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Histone H3 lysine 9 methylation and HP1gamma are associated with transcription elongation through mammalian chromatin.
Vakoc CR, Mandat SA, Olenchock BA, Blobel GA.
Molecular Cell 2005 Aug; 19(3):381.
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Long noncoding RNA NEAT1 suppresses hepatocyte proliferation in fulminant hepatic failure through increased recruitment of EZH2 to the LATS2 promoter region and promotion of H3K27me3 methylation.
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