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Last updated: 2017/7/23
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S100A1 polyclonal antibody

  • Catalog # : PAB12732
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against recombinant S100A1.
  • Immunogen:
  • Recombinant protein corresponding to human S100A1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 22, 11
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody recognizes 22 KDa for non-reduced S100A1 and 11 KDa for reduced S100A1.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (1:300)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing. Upon initial thawing, apportion into working aliquots and store at -80°C for up to six months.
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human melanoma with S100A1 polyclonal antibody (Cat # PAB12732) at 1 : 300 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6271
  • Gene Name:
  • S100A1
  • Gene Alias:
  • S100,S100-alpha,S100A
  • Gene Description:
  • S100 calcium binding protein A1
  • Gene Summary:
  • The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000035100,S100 alpha,S100 calcium-binding protein A1,S100 protein, alpha polypeptide
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