ROCK2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ROCK2.
Immunogen
A synthetic peptide corresponding to the epitope -VETFP- without a phosphorylation site surrounding T396 of human ROCK2.
Host
Rabbit
Theoretical MW (kDa)
165
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~165 KDa of human ROCK2 without a phosphorylation site of Thr396. It does not react to phosphorylated protein.
Form
Liquid
Purification
Epitope affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (0.01-0.1 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Western Blot (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (antibody stabilizer).
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunoprecipitation
The cell lysate derived from NIH/3T3 was immunoprecipitated by ROCK2 polyclonal antibody (Cat # PAB12730), then immunoprobed by the same antibody at 1 : 500 (lane 1). Lane 2 is a negative control.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ROCK2
Entrez GeneID
9475Gene Name
ROCK2
Gene Alias
KIAA0619
Gene Description
Rho-associated, coiled-coil containing protein kinase 2
Omim ID
604002Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq
Other Designations
OTTHUMP00000195104
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Interactome
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Pathway
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Disease
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Publication Reference
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Functional effects of rho-kinase-dependent phosphorylation of specific sites on cardiac troponin.
Vahebi S, Kobayashi T, Warren CM, de Tombe PP, Solaro RJ.
Circulation Research 2005 Mar; 96(7):740.
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Functional effects of rho-kinase-dependent phosphorylation of specific sites on cardiac troponin.
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