RHO polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The cell lysate derived from MCF-7 was immunoprobed by RHO polyclonal antibody (Cat # PAB12727) at 1 : 500. An immunoreactive band is observed around ~24 kDa (2). This band is abolished by pre-incubation with immunizing peptide (1).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RHO.
Immunogen
A synthetic peptide corresponding to C-terminus of human RHO.
Host
Rabbit
Theoretical MW (kDa)
24
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~24 KDa of human RHO.
Form
Liquid
Isotype
IgG
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The cell lysate derived from MCF-7 was immunoprobed by RHO polyclonal antibody (Cat # PAB12727) at 1 : 500. An immunoreactive band is observed around ~24 kDa (2). This band is abolished by pre-incubation with immunizing peptide (1).Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — RHO
Entrez GeneID
6010Gene Name
RHO
Gene Alias
CSNBAD1, MGC138309, MGC138311, OPN2, RP4
Gene Description
rhodopsin
Omim ID
180380Gene Ontology
HyperlinkGene Summary
Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq
Other Designations
opsin 2, rod pigment|retinitis pigmentosa 4, autosomal dominant|rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant)
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Interactome
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Disease
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Publication Reference
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The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis.
Zavzavadjian JR, Couture S, Park WS, Whalen J, Lyon S, Lee G, Fung E, Mi Q, Liu J, Wall E, Santat L, Dhandapani K, Kivork C, Driver A, Zhu X, Chang MS, Randhawa B, Gehrig E, Bryan H, Verghese M, Maer A, Saunders B, Ning Y, Subramaniam S, Meyer T, Simon MI, O'Rourke N, Chandy G, Fraser ID.
Molecular & Cellular Proteomics 2007 Mar; 6(3):413.
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The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis.
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