PTEN polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with PTEN polyclonal antibody (Cat # PAB12725) (Lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (Lane 2).
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : PTEN (pS385) (Phosphospecific)
B : PTEN (Paired S385) (Non phosphospeicfic)
C : Non-related phosphospecific peptide
were blotted at a 1 : 2000 dilution by :
1 : Phospho-PTEN S385 polyclonal antibody (Cat # PAB12751),
2 : PTEN polyclonal antibody (Cat # PAB12725).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PTEN.
Immunogen
A synthetic peptide corresponding to residues surrounding S385 of human PTEN.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes PTEN (Paired S385) without a phosphorylation site at Serine 385. It does not cross-react with phosphospecific peptides.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with PTEN polyclonal antibody (Cat # PAB12725) (Lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (Lane 2).Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : PTEN (pS385) (Phosphospecific)
B : PTEN (Paired S385) (Non phosphospeicfic)
C : Non-related phosphospecific peptide
were blotted at a 1 : 2000 dilution by :
1 : Phospho-PTEN S385 polyclonal antibody (Cat # PAB12751),
2 : PTEN polyclonal antibody (Cat # PAB12725). -
Gene Info — PTEN
Entrez GeneID
5728Gene Name
PTEN
Gene Alias
10q23del, BZS, MGC11227, MHAM, MMAC1, PTEN1, TEP1
Gene Description
phosphatase and tensin homolog
Gene Ontology
HyperlinkGene Summary
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq
Other Designations
MMAC1 phosphatase and tensin homolog deleted on chromosome 10|OTTHUMP00000020032|mutated in multiple advanced cancers 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Cooperative phosphorylation of the tumor suppressor phosphatase and tensin homologue (PTEN) by casein kinases and glycogen synthase kinase 3beta.
Al-Khouri AM, Ma Y, Togo SH, Williams S, Mustelin T.
The Journal of Biological Chemistry 2005 Aug; 280(42):35195.
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Cooperative phosphorylation of the tumor suppressor phosphatase and tensin homologue (PTEN) by casein kinases and glycogen synthase kinase 3beta.
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