PAK4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The cell lysate derived from 293 cells was immunoprobed by PAK4 polyclonal antibody (Cat # PAB12721) at 1 : 500.
An immunoreactive band is observed around ~68kDa (lane 1).
This band is blocked by pre-incubation with immunizing peptide (Lane 2).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PAK4.
Immunogen
A synthetic peptide corresponding to internal domain of human PAK4.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes PAK4. It does not cross react to other PAK1, PAK2 or PAK3.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The cell lysate derived from 293 cells was immunoprobed by PAK4 polyclonal antibody (Cat # PAB12721) at 1 : 500.
An immunoreactive band is observed around ~68kDa (lane 1).
This band is blocked by pre-incubation with immunizing peptide (Lane 2).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — PAK4
Entrez GeneID
10298Gene Name
PAK4
Gene Alias
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Gene Description
p21 protein (Cdc42/Rac)-activated kinase 4
Omim ID
605451Gene Ontology
HyperlinkGene Summary
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
p21(CDKN1A)-activated kinase 4|p21-activated kinase 4|protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
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Interactome
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Pathway
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Disease
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Publication Reference
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Requirement for PAK4 in the anchorage-independent growth of human cancer cell lines.
Callow MG, Clairvoyant F, Zhu S, Schryver B, Whyte DB, Bischoff JR, Jallal B, Smeal T.
J Biol Chem 2001 Oct; 277(1):550.
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Requirement for PAK4 in the anchorage-independent growth of human cancer cell lines.
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