The cell lysate derived from 293 cells was immunoprobed by PAK4 polyclonal antibody (Cat # PAB12721) at 1 : 500. An immunoreactive band is observed around ~68kDa (lane 1). This band is blocked by pre-incubation with immunizing peptide (Lane 2).
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
p21(CDKN1A)-activated kinase 4,p21-activated kinase 4,protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs