Rabbit polyclonal antibody raised against synthetic peptide of MMP8.
A synthetic peptide corresponding to the hinge region of human MMP8.
Theoretical MW (kDa):
This antibody recognizes ~65 KDa of MMP8.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (2-5 ug/mL) The optimal working dilution should be determined by the end user.
In TBS, pH 7.2 (BSA, 10% Proclin300)
Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. Upon initial thawing, apportion into working aliquots and store at -80°C for up to six months.
The whole cell lysate derived from SJCRH30 (human myosacorma) was resolved on 10% SDS-PAGE, and transferred onto NC membrane, and probed by MMP8 polyclonal antibody (Cat # PAB12713) at 1 : 100. An immunoreactive band at ~65 kDa was observed (A). This immunoreactive band is blocked by pre-incubation with the immunizing peptide (B).
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq