The tissue lysates derived from mouse liver were immunoblotted by MAP2K5 polyclonal antibody (Cat # PAB12705) at 1 : 1000 (lane 1). The lane 2 was a negative control.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane. A : MAP2K5 (pS311/pT315). B : MAP2K5 (Non-phospho). C : Non-related phospho-specific phosphopeptide. Followed were blotted at a 1 : 1000 dilution by : 1. Phospho-MAP2K5 S311/T315 polyclonal antibody (Cat # PAB12634). 2. MAP2K5 polyclonal antibody (Cat # PAB12705).
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq