LRP6 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The cell lysate derived from NIH/3T3 was immunoprobed by LRP6 polyclonal antibody (Cat # PAB12700) at 1 : 500. An immunoreactive band is observed around ~180kDa (1). This band is abolished by pre-incubation with immunizing peptide (2).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LRP6.
Immunogen
A synthetic peptide corresponding to amino acids (KGTYF) of human LRP6.
Host
Rabbit
Theoretical MW (kDa)
180
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~180 KDa of human LRP6.
Form
Liquid
Isotype
IgG
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The cell lysate derived from NIH/3T3 was immunoprobed by LRP6 polyclonal antibody (Cat # PAB12700) at 1 : 500. An immunoreactive band is observed around ~180kDa (1). This band is abolished by pre-incubation with immunizing peptide (2).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — LRP6
Entrez GeneID
4040Gene Name
LRP6
Gene Alias
ADCAD2, FLJ90062, FLJ90421
Gene Description
low density lipoprotein receptor-related protein 6
Gene Ontology
HyperlinkGene Summary
The LRP6 gene encodes a member of the low density lipoprotein receptor (LDLR) gene family, which consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDLR proteins are comprised of the same basic structural motifs: an extracellular domain that contains LDLR binding repeats and EGF repeats with associated spacer domains containing the YWTD motif; a single transmembrane-spanning domain; and a C-terminal cytoplasmic domain that generally contains at least 1 copy of the NPXY motif (Brown et al., 1998 [PubMed 9704021]). LRP6 and LRP5 (MIM 603506) function as coreceptors for WNT (MIM 164820) ligands and thus play a role in WNT signaling, which is important in a wide variety of biologic processes during pre- and postnatal life in invertebrates and vertebrates (Kokubu et al., 2004 [PubMed 15469977]).[supplied by OMIM
Other Designations
low density lipoprotein-related protein 6
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Interactome
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Pathway
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Disease
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Publication Reference
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Analysis of endogenous LRP6 function reveals a novel feedback mechanism by which Wnt negatively regulates its receptor.
Khan Z, Vijayakumar S, de la Torre TV, Rotolo S, Bafico A.
Molecular and Cellular Biology 2007 Oct; 27(20):7291.
Application：IP, WB, Human, Monkey, Mouse, COS-7, HEK 293T, NIH 3T3 cells.
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Analysis of endogenous LRP6 function reveals a novel feedback mechanism by which Wnt negatively regulates its receptor.
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