LRP5 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LRP5.
Immunogen
A synthetic peptide corresponding to amino acids (KATLY) of human LRP5.
Host
Rabbit
Theoretical MW (kDa)
175
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~175 KDa of human LRP5.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The cell lysate derived from NIH/3T3 was immunoprobed by LRP5 polyclonal antibody (Cat # PAB12699) at 1 : 500. An immunoreactive band is observed around ~175 kDa (1). This band is abolished by pre-incubation with immunizing peptide (2).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — LRP5
Entrez GeneID
4041Gene Name
LRP5
Gene Alias
BMND1, EVR1, EVR4, HBM, LR3, LRP7, OPPG, OPS, OPTA1, VBCH2
Gene Description
low density lipoprotein receptor-related protein 5
Gene Ontology
HyperlinkOther Designations
exudative vitreoretinopathy 1|low density lipoprotein receptor-related protein 7|osteoporosis pseudoglioma syndrome
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Interactome
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Pathway
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Disease
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Publication Reference
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Analysis of endogenous LRP6 function reveals a novel feedback mechanism by which Wnt negatively regulates its receptor.
Khan Z, Vijayakumar S, de la Torre TV, Rotolo S, Bafico A.
Molecular and Cellular Biology 2007 Oct; 27(20):7291.
Application:WB, Human, HEK 293T cells.
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Analysis of endogenous LRP6 function reveals a novel feedback mechanism by which Wnt negatively regulates its receptor.
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