Rabbit polyclonal antibody raised against synthetic peptide of MKI67.
A synthetic peptide corresponding to human MKI67.
Theoretical MW (kDa):
Human, Mouse, Rat
This antibody recognizes ~67 KDa of MKI67.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (2-5 ug/mL) The optimal working dilution should be determined by the end user.
In TBS, pH 7.2 (BSA, 10% Proclin300)
Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. Upon initial thawing, apportion into working aliquots and store at -80°C for up to six months.
Human lymph node stained with MKI67 polyclonal antibody (Cat # PAB12698) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq