IRS1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of IRS1.
Immunogen
A synthetic peptide corresponding to the intra domain of human IRS1.
Host
Rabbit
Theoretical MW (kDa)
165
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~165 KDa of human IRS1.
Form
Liquid
Purification
Epitope affinity purification
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (antibody stabilizer).
Storage Instruction
Store at 4°C for three month without detectable loss of activity. For long term storage, store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon tissue was stained with IRS1 polyclonal antibody (Cat # PAB12694) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — IRS1
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Interactome
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Pathway
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Disease
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Publication Reference
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Protein kinase C Theta inhibits insulin signaling by phosphorylating IRS1 at Ser(1101).
Li Y, Soos TJ, Li X, Wu J, Degennaro M, Sun X, Littman DR, Birnbaum MJ, Polakiewicz RD.
The Journal of Biological Chemistry 2004 Sep; 279(44):45304.
Application:IP-WB, Mouse, 3T3-L1, C2C12 cells.
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Protein kinase C Theta inhibits insulin signaling by phosphorylating IRS1 at Ser(1101).
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