The nuclear extract derived from HeLa were immunoprecipitated by 4 ug of HIST2H3C polyclonal antibody (Cat # PAB12691), then probed with HIST2H3C polyclonal antibody at 1 : 1000. An immunoreactive band at ~17 KDa is observed. Note : ~50 KDa and ~22 KDa are the rabbit immunoglobulin heavy and light chains.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq
H3 histone family, member M,H3 histone, family 2,OTTHUMP00000014041,histone 2, H3c