FOXP3 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The HeLa cell lysate was immuno-blotted by FOXP3 polyclonal antibody (Cat # PAB12685) at 1 : 500 (Lane 1). Lane 2 was pre-incubated with immunizing peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis in human tonsil with FOXP3 polyclonal antibody (Cat # PAB12685) at 5 ug/mL.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of FOXP3.
Immunogen
A synthetic peptide corresponding to C-terminus of human FOXP3.
Host
Rabbit
Theoretical MW (kDa)
52
Reactivity
Human, Mouse, Rat
Specificity
This antibody only recognizes ~52 KDa of human FOXP3.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (antibody stabilizer)
Storage Instruction
Store at 4°C. For long term storage store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The HeLa cell lysate was immuno-blotted by FOXP3 polyclonal antibody (Cat # PAB12685) at 1 : 500 (Lane 1). Lane 2 was pre-incubated with immunizing peptide.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis in human tonsil with FOXP3 polyclonal antibody (Cat # PAB12685) at 5 ug/mL.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — FOXP3
Entrez GeneID
50943Gene Name
FOXP3
Gene Alias
AIID, DIETER, IPEX, JM2, MGC141961, MGC141963, PIDX, XPID
Gene Description
forkhead box P3
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000025832|immune dysregulation, polyendocrinopathy, enteropathy, X-linked|immunodeficiency, polyendocrinopathy, enteropathy, X-linked|scurfin
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Interactome
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Disease
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Publication Reference
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Cooperative regulation in development by SMRT and FOXP1.
Jepsen K, Gleiberman AS, Shi C, Simon DI, Rosenfeld MG.
Genes & Development 2008 Mar; 22(6):740.
Application：ChIP, Human, Mouse heart, THP-1 cells.
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Cooperative regulation in development by SMRT and FOXP1.
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