ESR2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ESR2.
Immunogen
A synthetic peptide corresponding to C-terminus of human ESR2.
Host
Rabbit
Theoretical MW (kDa)
53
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~53 KDa of human ESR2.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The whole cell lysates derived from MCF-7 were immunoblotted by ESR2 polyclonal antibody (Cat # PAB12682) at 1 : 500 (Lane 1). The immunoreactive band is blocked by pre-incubation of immunizing peptide (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue stained with ESR2 polyclonal antibody (Cat # PAB12682) at 1 : 200 for 10 min at RT. Staining of formalin-fixedtissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ESR2
Entrez GeneID
2100Gene Name
ESR2
Gene Alias
ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2
Gene Description
estrogen receptor 2 (ER beta)
Omim ID
601663Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq
Other Designations
estrogen receptor beta|estrogen receptor beta 4
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Interactome
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Disease
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Publication Reference
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Estrogen receptor (ER) beta, a modulator of ERalpha in the uterus.
Weihua Z, Saji S, Mäkinen S, Cheng G, Jensen EV, Warner M, Gustafsson JA.
PNAS 2000 May; 97(11):5936.
Application:IF, IHC-Fr, Mouse, Mouse uterus.
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Estrogen receptor (ER) beta, a modulator of ERalpha in the uterus.
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