The whole cell lysates derived from MCF-7 were immunoblotted by ESR2 polyclonal antibody (Cat # PAB12682) at 1 : 500 (Lane 1). The immunoreactive band is blocked by pre-incubation of immunizing peptide (Lane 2).
Immunohistochemical staining of human breast cancer tissue stained with ESR2 polyclonal antibody (Cat # PAB12682) at 1 : 200 for 10 min at RT. Staining of formalin-fixedtissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq