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Last updated: 2016/10/23
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HIST2H3C polyclonal antibody

  • Catalog # : PAB12679
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of HIST2H3C.
  • Immunogen:
  • A synthetic peptide corresponding to dimethyl lysine 27 of human HIST2H3C.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 17
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody only recognizes ~17 KDa of Dimethylated HIST2H3C. It does not cross react to non-methylated H3 or mono-, Tri-methylated Histone H3.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (1:100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • ChIP
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Fluorescent Immunohistochemistry : NIH/3T3 cells were cultured in chamber, and fixed onto slide followed by incubation with 5 ug/mL HIST2H3C polyclonal antibody (Cat # PAB12679), then FITC labeled Goat anti Rabbit IgG for visualization under fluorescent microscope.
  • Immunoprecipitation
  • Immunoprecipitation
  • The nuclear extract derived from NIH/3T3 were immunoprecipitated by 4 ug of HIST2H3C polyclonal antibody (Cat # PAB12679), then probed with HIST2H3C polyclonal antibody (Cat # PAB12679) at 1 : 1000. An immunoreactive band at ~17 KDa is observed.
    Note : ~50 KDa and ~22 KDa are the rabbit immunoglobulin heavy and light chain.
  • Application Image
  • ChIP
  • Western Blot
  • Immunohistochemistry
  • Gene Information
  • Gene Name:
  • HIST2H3C
  • Gene Alias:
  • H3,H3.2,H3/M,H3F2,H3FM,MGC9629
  • Gene Description:
  • histone cluster 2, H3c
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq
  • Other Designations:
  • H3 histone family, member M,H3 histone, family 2,OTTHUMP00000014041,histone 2, H3c
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