HIST2H3C polyclonal antibody
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More Files
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Immunofluorescence
Fluorescent Immunohistochemistry : NIH/3T3 cells were cultured in chamber, and fixed onto slide followed by incubation with 5 ug/mL HIST2H3C polyclonal antibody (Cat # PAB12679), then FITC labeled Goat anti Rabbit IgG for visualization under fluorescent microscope.
Immunoprecipitation
The nuclear extract derived from NIH/3T3 were immunoprecipitated by 4 ug of HIST2H3C polyclonal antibody (Cat # PAB12679), then probed with HIST2H3C polyclonal antibody (Cat # PAB12679) at 1 : 1000. An immunoreactive band at ~17 KDa is observed.
Note : ~50 KDa and ~22 KDa are the rabbit immunoglobulin heavy and light chain.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HIST2H3C.
Immunogen
A synthetic peptide corresponding to dimethyl lysine 27 of human HIST2H3C.
Host
Rabbit
Theoretical MW (kDa)
17
Reactivity
Human, Mouse, Rat
Specificity
This antibody only recognizes ~17 KDa of Dimethylated HIST2H3C. It does not cross react to non-methylated H3 or mono-, Tri-methylated Histone H3.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
ChIP
Western Blot
Immunohistochemistry
Immunofluorescence
Fluorescent Immunohistochemistry : NIH/3T3 cells were cultured in chamber, and fixed onto slide followed by incubation with 5 ug/mL HIST2H3C polyclonal antibody (Cat # PAB12679), then FITC labeled Goat anti Rabbit IgG for visualization under fluorescent microscope.Immunoprecipitation
The nuclear extract derived from NIH/3T3 were immunoprecipitated by 4 ug of HIST2H3C polyclonal antibody (Cat # PAB12679), then probed with HIST2H3C polyclonal antibody (Cat # PAB12679) at 1 : 1000. An immunoreactive band at ~17 KDa is observed.
Note : ~50 KDa and ~22 KDa are the rabbit immunoglobulin heavy and light chain.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HIST2H3C
Entrez GeneID
126961Gene Name
HIST2H3C
Gene Alias
H3, H3.2, H3/M, H3F2, H3FM, MGC9629
Gene Description
histone cluster 2, H3c
Omim ID
142780Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq
Other Designations
H3 histone family, member M|H3 histone, family 2|OTTHUMP00000014041|histone 2, H3c
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Interactome
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Pathway
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Publication Reference
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Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins.
Seward DJ, Cubberley G, Kim S, Schonewald M, Zhang L, Tripet B, Bentley DL.
Nature Structural & Molecular Biology 2007 Feb; 14(3):240.
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Histone H3 lysine 9 methylation and HP1gamma are associated with transcription elongation through mammalian chromatin.
Vakoc CR, Mandat SA, Olenchock BA, Blobel GA.
Molecular Cell 2005 Aug; 19(3):381.
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Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins.
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