Rabbit polyclonal antibody raised against synthetic peptide of KRT19.
Immunogen:
A synthetic peptide corresponding to N-terminus of human KRT19.
Host:
Rabbit
Theoretical MW (kDa):
40
Reactivity:
Human, Mouse
Specificity:
This antibody only recognizes ~40 KDa of human KRT19.
Form:
Liquid
Purification:
Affinity Purification
Isotype:
IgG
Recommend Usage:
Flow Cytometry ELISA (0.01-0.1 ug/mL) Immunohistochemistry (0.5-2 ug/mL) Immunoprecipitation (2-5 ug/mL) Western Blot (0.1-1 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.2 (antibody stabilizer).
Storage Instruction:
Store at 4°C. For long term storage store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
The whole cell lysate derived from SK-BR-3 cell was immunoprobed by KRT19 polyclonal antibody (Cat # PAB12676) at 1:500. An immunoreactive band is observed around ~40kDa.
Mouse liver cell lysate was stained with KRT19 polyclonal antibody (Cat# PAB12676) at 1:500 for 10 min RT. Staining of formalinfixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq
Other Designations:
40-kDa keratin intermediate filament,cytokeratin 19,keratin, type I cytoskeletal 19,keratin, type I, 40-kd